The recommended levels when used with an anti-androgen are 100-200 pg/ml at trough.

The recommended levels for estradiol monotherapy are 250-350 pg/ml at trough.

Targeting the lowest levels that properly suppress testosterone while having positive psychological and physiological effects is recommended, as higher estradiol levels are linked to an increased risk of clotting and cancer.

Data labelled Author's Tests is taken from blood tests done by the author, plus some estimations using math plus some reference data from Wikipedia.

Data labelled 3rd Party Tests is provided by volunteer blood tests and processed in the same way as the author's.

Data labelled Studies is taken from medical studies, many of them aggregated via Wikipedia or Transfeminine Science.